A Case Report of Germline Compound Heterozygous Mutations in the BRCA1 Gene of an Ovarian and Breast Cancer Patient

The germline carrier of the BRCA1 pathogenic mutation has been well proven to confer an increased risk of breast and ovarian cancer. Despite BRCA1 biallelic pathogenic mutations being extremely rare, they have been reported to be embryonically lethal or to cause Fanconi anemia (FA). Here we describe a patient who was a 48-year-old female identified with biallelic pathogenic mutations of the BRCA1 gene, with no or very subtle FA-features. She was diagnosed with ovarian cancer and breast cancer at the ages of 43 and 44 and had a strong family history of breast and gynecological cancers.     

平行坐标技术在干式变压器温度场可视化分析中的应用

本文中笔者将平行坐标技术应用于变压器温度场多维可视化分析,以矿用变压器为例,给出了其高、低压绕组纵向温度分布.     

建筑垃圾处理现状及再利用措施

我国城市化发展进程不断加快,各个地区建筑数量不断增多,规模不断扩大,在发展的同时,建筑行业产生的建筑垃圾数量也十分惊人。这些建筑垃圾不但包括施工过程中产生的废弃料,还包括拆除旧建筑产生的固体垃圾。如果没有妥善处理这些建筑垃圾,必然会对城市土地、空气、水源等产生一定的不良影响。为此,相关工作人员需要明确我国建筑垃圾处理现状,加强分析建筑垃圾资源化利用的方式,并且不断优化建筑垃圾处理工艺,提高垃圾资利用水平。     

Synthesis,Anticancer Activity,Structure-Activity Relationship and Mechanistic Investigations of Falcarindiol Analogues

Forty samples of optically active falcarindiol analogues are synthesized by using the easily available C2 symmetric (R)- and (S)-1,1’-binaphth-2-ol (BINOL) in combination with Ti(OⁱPr)₄, Zn powder and EtI. Their anticancer activities on Hccc-9810, HepG2, MDA-MB-231, Hela, MG-63 and H460 cells are assayed to elucidate their structure-activity relationships. These results showed that the falcarindiol analogue (3R,8S)- 2 i with the terminal double bond has the most potent anti-proliferation effect on Hccc-9810 cells with IC₅₀ value of 0.46 μM. The falcarindiol analogue (3R,8S)- 2 i can induce obvious Hccc-9810 cell apoptosis in a concentration-dependent manner by Hoechst staining and flow cytometry analysis. The proposed mechanism suggests that the falcarindiol analogue (3R,8S)- 2 i increases LDH release and MDA content, and reduces the levels of SOD activity, which lead to the accumulation of oxidative stress and induce apoptosis in Hccc-9810 cells.     

Lactobacillus plantarum ZLC-18 fermentation improve tyrosinase inhibition activity and antioxidant capacity in soybean hulls

Soybean hulls, the main byproduct in soybean processing, have many biological activities. However, the value of this byproduct is still far from being fully exploited. In this study, we investigated the anti-tyrosinase and antioxidant capacities in soybean hulls fermented by Lactobacillus plantarum ZLC-18 (L. plantarum ZLC-18). We found that L. plantarum ZLC-18 fermentation could markedly improve the anti-tyrosinase and antioxidant activities of soybean hulls. The anti-tyrosinase and antioxidant activities were positively correlated with the total phenolic and flavonoid contents. UPLC–MS analysis suggested that phenolic compounds (daidzin, genistin, vanillic acid and so on) were increased in soybean hulls after fermentation, and some of these compounds were shown to have anti-tyrosinase and antioxidant activities. Our study demonstrates that fermentation is an efficient strategy to enhance the bioactive function of soybean hulls. Moreover, this study provides evidence that soybean hulls can be used as a kind of functional food ingredient, thereby improving their economic benefits.     

4'-氯-2-氨基联苯的绿色合成与表征

以对氯溴苯和邻氯硝基苯为原料,经格氏反应、Suzuki偶联、Pd-C催化加氢还原3步反应合成了4'-氯-2-氨基联苯.格氏反应中,优化的反应条件为n(对氯溴苯)∶n(硼酸三甲酯)=1.0∶1.2,反应温度为0℃.Suzuki偶联反应中,优化的反应条件为n(邻氯硝基苯)∶n(4-氯苯硼酸)∶n(PdCl2(PPh3)2)=1.00∶1.10∶0.01,还原反应的较佳反应温度为50℃.在优化的反应条件下,4'-氯-2-氨基联苯的合成总收率≥59.7％(以对氯溴苯计),纯度95.0％;产物结构经IR、1H NMR、MS确证.该工艺简单经济,条件温和,高效环保,适合工业化生产.     

基于半监督卷积神经网络的心律不齐分类

心律不齐早期的检测和分类有十分重要的价值.然而,由于心律不齐特征不够明显、不同人体心电信号存在较大的差异性等问题,心律不齐分类技术在现实中的应用存在很大困难.针对上述问题,提出了一种基于半监督卷积神经网络的心律不齐分类方法.首先,对心电信号进行马尔可夫变迁场变换将其变为二维图像;然后,迁移二维卷积神经网络训练得到普通模型;最后,使用半监督训练的方法得到特定模型,使用得到的特定模型对特定病人的心电信号进行分类.该方法从二维图像中提取更高层次的特征来提高分类性能,并有效地克服不同人体心电信号之间的差异性,增强了模型的泛化能力.遵循美国医疗器械促进协会标准,使用麻省理工学院和波士顿贝丝以色列医院心律不齐数据库中的数据,完成了心律不齐五分类,总准确率达到了97.9％,提升了心律不齐分类技术的现实应用价值.     

棉籽油的深度分提工艺

新疆棉籽油含有700 mg/kg的蜡质和26%～28%的固体脂，由于新疆气温多在零度以下，棉籽油在运输、储存和销售过程中易产生固化、沉淀、发朦，影响棉籽油的外观、食用和销售。为了保持包装棉籽油透明，提高棉籽油的质量和附加值，对棉籽油进行深度分提以脱蜡脱脂。介绍了棉籽油深度分提工艺，即在15 ℃脱除蜡质，在7 ℃分提出棕榈酸甘三酯，继续降温至-10 ℃分提出棕榈酸甘二酯、棕榈酸甘一酯及其他硬脂，最终得到在低温下保持透明的棉籽液油。